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What Does the Research Say about Ozone Therapy? 

Clean Bubbles

A Legacy of Healing, A Future of Wellness

For over 100 years, ozone therapy has been safely used by practitioners around the world. As a therapy that uses a naturally occurring molecule—a special form of oxygen—it works by stimulating the body's own innate healing mechanisms.

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Because natural substances cannot be patented, ozone therapy has not received the extensive funding from pharmaceutical companies that is typically required for large-scale studies. As a result, much of the current evidence comes from promising preclinical and smaller human trials that highlight its effectiveness.

 

This places it in a category of powerful, natural therapies whose benefits are recognized through decades of successful clinical use and a growing body of international research.

We believe in being fully transparent about the evidence and are committed to practicing this therapy based on its long history of safety and the positive outcomes demonstrated in international research and clinical practice.
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🔥 Ozone & Inflammation

Early research suggests ozone therapy may:
✅ Activate the body’s natural antioxidant defenses
✅ Lower inflammatory signals (like TNF-α, IL-1β, IL-6)
✅ Increase protective anti-inflammatory markers (like IL-10)
✅ Reduce joint damage and improve redox balance in studies​​

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🎗️ Ozone & Cancer Care

Early research suggests ozone therapy may:
✅ Boost the effect of certain chemotherapy drugs
✅ Reduce inflammation
✅ Improve oxygen flow in the body
✅ Lessen side effects, helping you feel better during treatment

⚠️ Note: Ozone is not a stand-alone cancer treatment. It may be used alongside standard care to support your body.

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🛡️ Ozone & Immune Function

Early research suggests ozone therapy may:
✅ Balance the immune system by restoring antioxidant defenses
✅ Reduce harmful pro-inflammatory signals (like TNF-α, IL-6)
✅ Boost protective anti-inflammatory signals (like IL-10)
✅ Support regulatory T cells (Tregs) that calm immune overreactions
✅ Improve immune balance in some chronic and infectious conditions

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💙 Ozone & Chronic Diseases

Early studies suggest ozone therapy may help in conditions like type 2 diabetes and cardiovascular disease by:
✅ Improving antioxidant defenses and reducing oxidative stress
✅ Lowering harmful blood markers linked to inflammation and heart risk
✅ Supporting wound healing in diabetic foot ulcers, with fewer amputations reported in trials
✅ Improving blood flow and clotting balance in patients with coronary artery disease

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Note: Most information comes from small studies, larger human trials are still needed to really determine the effects of ozone therapies. ​

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References

Modulation by Ozone Therapy of Oxidative Stress in Chemotherapy-Induced Peripheral Neuropathy: The Background for a Randomized Clinical Trial.

Clavo B, Martínez-Sánchez G, Rodríguez-Esparragón F, et al.

International Journal of Molecular Sciences. 2021;22(6):2802. doi:10.3390/ijms22062802.

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Ozone Treatment in the Management of Chemotherapy-Induced Peripheral Neuropathy: A Review of Rationale and Research Directions.

Clavo B, Cánovas-Molina A, Federico M, et al.

Cancers. 2025;17(14):2278. doi:10.3390/cancers17142278.

 New Research

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Long-Term Improvement by Ozone Treatment in Chronic Pain Secondary to Chemotherapy-Induced Peripheral Neuropathy: A Preliminary Report.

Clavo B, Rodríguez-Abreu D, Galván S, et al.

Frontiers in Physiology. 2022;13:935269. doi:10.3389/fphys.2022.935269.

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Long-Term Effects of Ozone Treatment in Patients With Persistent Numbness and Tingling Secondary to Chemotherapy-Induced Peripheral Neuropathy. A Retrospective Study.

Clavo B, Rodríguez-Abreu D, Galván-Ruiz S, et al.

Integrative Cancer Therapies. 2025 Jan-Dec;24:15347354241307038. doi:10.1177/15347354241307038.

 New Research

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Combination ozone therapy as an effective method of radiomodification in chemoradiation treatment of patients with cervical cancer.

Rodionova O, Gusareva M, Sheiko E, et al.

Journal of Clinical Oncology. 2021;39(Suppl 15):e17515. doi:10.1200/JCO.2021.39.15_suppl.e17515.

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Ozone in Medicine. The Low-Dose Ozone Concept and Its Basic Biochemical Mechanisms of Action in Chronic Inflammatory Diseases.

Viebahn-Haensler R, León Fernández OS.

International Journal of Molecular Sciences. 2021;22(15):7890. doi:10.3390/ijms22157890.

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Low-Dose Ozone as a Eustress Inducer: Experimental Evidence of the Molecular Mechanisms Accounting for Its Therapeutic Action.

Malatesta M, Tabaracci G, Pellicciari C.

International Journal of Molecular Sciences. 2024;25(23):12657. doi:10.3390/ijms252312657.

 New Research

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Oxygen-Ozone Therapy for Reducing Pro-Inflammatory Cytokines Serum Levels in Musculoskeletal and Temporomandibular Disorders: A Comprehensive Review.

de Sire A, Marotta N, Ferrillo M, et al.

International Journal of Molecular Sciences. 2022;23(5):2528. doi:10.3390/ijms23052528.

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Anti-Inflammatory Effect of Ozone Therapy in an Experimental Model of Rheumatoid Arthritis.

Tartari APS, Moreira FF, Pereira MCDS, et al.

Inflammation. 2020;43(3):985-993. doi:10.1007/s10753-020-01184-2.

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Mitochondrial Dysfunction, Its Oxidative Stress-Induced Pathologies and Redox Bioregulation Through Low-Dose Medical Ozone: A Systematic Review.

Viebahn-Haensler R, León Fernández OS.

Molecules (Basel, Switzerland). 2024;29(12):2738. doi:10.3390/molecules29122738.

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Ozone Oxidative Postconditioning Ameliorates Joint Damage and Decreases Pro-Inflammatory Cytokine Levels and Oxidative Stress in PG/­PS-induced Arthritis in Rats.

Vaillant JD, Fraga A, Díaz MT, et al.

European Journal of Pharmacology. 2013;714(1-3):318-24. doi:10.1016/j.ejphar.2013.07.034.

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Medical Ozone Increases Methotrexate Effects in Rheumatoid Arthritis Through a Shared New Mechanism Which Involves Adenosine.

León Fernández OS, Oru GT, Viebahn-Haensler R, et al.

International Journal of Molecular Sciences. 2025;26(11):5256. doi:10.3390/ijms26115256.

 New Research

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Medical Ozone Promotes Nrf2 Phosphorylation Reducing Oxidative Stress and Pro-Inflammatory Cytokines in Multiple Sclerosis Patients.

Delgado-Roche L, Riera-Romo M, Mesta F, et al.

European Journal of Pharmacology. 2017;811:148-154. doi:10.1016/j.ejphar.2017.06.017.

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Ozone as Redox Bioregulator in Preventive Medicine: The Molecular and Pharmacological Basis of the Low-Dose Ozone Concept-a Review.

Viebahn-Haensler R, León Fernández OS.

International Journal of Molecular Sciences. 2023;24(21):15747. doi:10.3390/ijms242115747.

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Low-Dose Ozone as a Eustress Inducer: Experimental Evidence of the Molecular Mechanisms Accounting for Its Therapeutic Action.

Malatesta M, Tabaracci G, Pellicciari C.

International Journal of Molecular Sciences. 2024;25(23):12657. doi:10.3390/ijms252312657.

 New Research

​

Ozone in Medicine. The Low-Dose Ozone Concept and Its Basic Biochemical Mechanisms of Action in Chronic Inflammatory Diseases.

Viebahn-Haensler R, León Fernández OS.

International Journal of Molecular Sciences. 2021;22(15):7890. doi:10.3390/ijms22157890.

​

Mitochondrial Dysfunction, Its Oxidative Stress-Induced Pathologies and Redox Bioregulation Through Low-Dose Medical Ozone: A Systematic Review.

Viebahn-Haensler R, León Fernández OS.

Molecules (Basel, Switzerland). 2024;29(12):2738. doi:10.3390/molecules29122738.

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The Effects of Oxygen-Ozone Therapy on Regulatory T-Cell Responses in Multiple Sclerosis Patients.

Tahmasebi S, Qasim MT, Krivenkova MV, et al.

Cell Biology International. 2021;45(7):1498-1509. doi:10.1002/cbin.11589.

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Anti-Inflammatory Effect of Ozone Therapy in an Experimental Model of Rheumatoid Arthritis.

Tartari APS, Moreira FF, Pereira MCDS, et al.

Inflammation. 2020;43(3):985-993. doi:10.1007/s10753-020-01184-2.

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Ozone Therapy Restores Immune Dysfunction in Refractory Idiopathic Granulomatous Mastitis as a Novel Potential Therapeutic Approach.

Cabioglu N, Cetin Aktas E, Emiroglu S, et al.

Cell Biology International. 2023;47(1):228-237. doi:10.1002/cbin.11953.

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Mechanisms of Action of Ozone Therapy in Emerging Viral Diseases: Immunomodulatory Effects and Therapeutic Advantages With Reference to SARS-CoV-2.

Cenci A, Macchia I, La Sorsa V, et al.

Frontiers in Microbiology. 2022;13:871645. doi:10.3389/fmicb.2022.871645.

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Ozone Therapy in COVID-19: A Narrative Review.

Cattel F, Giordano S, Bertiond C, et al.

Virus Research. 2021;291:198207. doi:10.1016/j.virusres.2020.198207.

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Therapeutic Efficacy of Ozone in Patients With Diabetic Foot.

Martínez-Sánchez G, Al-Dalain SM, Menéndez S, et al.

European Journal of Pharmacology. 2005;523(1-3):151-61. doi:10.1016/j.ejphar.2005.08.020.

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A New Treatment Strategy That Reduces Growth/­Differentiation Factor-15 in Type-2 Diabetes Mellitus: Major Autohemotherapy: A Pilot Study.

Yücel HE, İlanbey B. A

Alternative Therapies in Health and Medicine. 2023;29(7):30-33.

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The Effect of Gaseous Ozone Therapy in Conjunction With Periodontal Treatment on Glycated Hemoglobin Level in Subjects With Type 2 Diabetes Mellitus: An Unmasked Randomized Controlled Trial.

Rapone B, Ferrara E, Corsalini M, et al.

International Journal of Environmental Research and Public Health. 2020;17(15):E5467. doi:10.3390/ijerph17155467.

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Effects of Ozone Therapy on Haemostatic and Oxidative Stress Index in Coronary Artery Disease.

Martínez-Sánchez G, Delgado-Roche L, Díaz-Batista A, Pérez-Davison G, Re L.

European Journal of Pharmacology. 2012;691(1-3):156-62. doi:10.1016/j.ejphar.2012.07.010.

 

5.

Medical Ozone Arrests Oxidative Damage Progression and Regulates Vasoactive Mediator Levels in Elderly Patients (60-70 years) With Oxidative Etiology Diseases.

León Fernández OS, Oru GT, Viebahn-Hänsler R, et al.

Frontiers in Physiology. 2022;13:1029805. doi:10.3389/fphys.2022.1029805.

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